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KMID : 0043319910140030207
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Archives of Pharmacal Research
1991 Volume.14 No. 3 p.207 ~ p.216
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Comparison of Lecfin from Pseudixus japonicus and Concanavalin A on Lymphocytes Proliferation and Cytotoxicity
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Chung YZ
Jung HO/Hong GT/Suh SS
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Abstract
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Pseudixus japonicus agglutinin (PJA) was isolated. And its characteristics were compared with those of concanavalin A (Con A). PJA is a glycoprotein composed of 49.3% carbohydrate and 50.7% protein which had relatively high percentages ol’’ glutamic acid, aspartic acid and phenylalanine residues. The hemagglutinating activity of PJA was approximately one-eighth of that of Con A when tested with mouse erythrocytes. PJA failed to stimulate the proliferation or transformation of human and mouse lymphocytes in contrast to Con A. PJA and Con A showed cytotoxicities against SNU-1 (human stomach cancer cells), SNU-C1 (human colon cancer cells) and mouse Sarcoma 180 cells when tested by 3-(4,5-dimethyl thiazol-2-yl)-2-5-diphenyl tetrazolium bromide (M-FT) colorimetric assay. The antitumor activity of the lectin in vitro was also tested in Sarcoma 180 bearing mice. There was no significant difference in prolongation of life spun of the mice after the treatment with PJA and Con A for 10 Consecutive days.
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KEYWORD
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